primary human skeletal muscle derived cells hskmdcs  (Cook MyoSite Inc)

Journal: Biomedicines

Article Title: Developing In Vitro Models to Define the Role of Direct Mitochondrial Toxicity in Frequently Reported Drug-Induced Rhabdomyolysis

doi: 10.3390/biomedicines11051485

Figure Lengend Snippet: The effect of rotenone (0–5 µM, positive control) exposure on ATP levels and LDH retention in HSKMDC (2 h), compared to the vehicle control. HSKMDC cells were exposed to rotenone in glucose or galactose serum-free media. Statistical significance compared to the vehicle control was determined by one-way ANOVA with Dunnett’s correction for multiple comparisons. ATP galactose ^^ p -value < 0.01, ^^^ p -value < 0.001. ATP levels are reported as the percentage of the vehicle control and expressed as mean ± S.D. ( n = 3). Key: blue circles represent ATP glucose, orange squares represent ATP galactose, the black triangle represents cytotoxicity-HSKMDC–glucose, and the grey triangle down represents cytotoxicity–HSKMDC–galactose.

Article Snippet: Human primary skeletal muscle-derived cells (HSKMDC) (catalogue #SK-1111, Lot #P0100750F) were obtained from Cook Myosite Ltd. (Pittsburgh, PA, USA).

Techniques: Positive Control, Control

Journal: Biomedicines

Article Title: Developing In Vitro Models to Define the Role of Direct Mitochondrial Toxicity in Frequently Reported Drug-Induced Rhabdomyolysis

doi: 10.3390/biomedicines11051485

Figure Lengend Snippet: Table reporting IC 50 values for each suspect drug tested in HSKMDC using the acute metabolic switch assay. Values were determined by fit spline and interpolating IC 50 concentrations from the standard curve. Results are displayed as mean ± S.D. ( n = 3). Statistical significance was determined by unpaired t -test with Welch’s correction.

Article Snippet: Human primary skeletal muscle-derived cells (HSKMDC) (catalogue #SK-1111, Lot #P0100750F) were obtained from Cook Myosite Ltd. (Pittsburgh, PA, USA).

Techniques:

Journal: Biomedicines

Article Title: Developing In Vitro Models to Define the Role of Direct Mitochondrial Toxicity in Frequently Reported Drug-Induced Rhabdomyolysis

doi: 10.3390/biomedicines11051485

Figure Lengend Snippet: The effect of ( A ) fenofibrate and ( B ) risperidone exposure (2 h) on ATP content and LDH retention in HSKMDC. Serial concentrations of the compounds were used up to 300 µM in glucose or galactose serum-free media. ATP and cytotoxicity levels are expressed as a percentage of the corresponding media vehicle control and values are displayed as mean ± S.D. ( n = 3). Statistical significance compared to the vehicle control was determined by one-way ANOVA with Dunnett’s correction for multiple comparisons. ATP glucose ++ p -value < 0.01. ATP galactose ^^ p -value < 0.01, ^^^ p -value < 0.001, ^^^^ p -value < 0.001. Key: blue circles represent ATP glucose, orange squares represent ATP galactose, the black triangle represents cytotoxicity-HSKMDC–glucose, and the grey triangle down represents cytotoxicity–HSKMDC–galactose.

Article Snippet: Human primary skeletal muscle-derived cells (HSKMDC) (catalogue #SK-1111, Lot #P0100750F) were obtained from Cook Myosite Ltd. (Pittsburgh, PA, USA).

Techniques: Control

Journal: Biomedicines

Article Title: Developing In Vitro Models to Define the Role of Direct Mitochondrial Toxicity in Frequently Reported Drug-Induced Rhabdomyolysis

doi: 10.3390/biomedicines11051485

Figure Lengend Snippet: Examining the effect of the acute injection of ( A ) fenofibrate and ( B ) risperidone on respiratory parameters in HSKMDC. Values are expressed as a percentage of vehicle control ± S.D. ( n = 3). The values were normalised to μg of protein per well. Statistical significance was determined by two-way analysis of variance (ANOVA) with Dunnett’s correction for multiple comparisons. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, **** p -value < 0.001.

Article Snippet: Human primary skeletal muscle-derived cells (HSKMDC) (catalogue #SK-1111, Lot #P0100750F) were obtained from Cook Myosite Ltd. (Pittsburgh, PA, USA).

Techniques: Injection, Control